Honey bee brood development

The study is performed usually in field conditions or in semi-field conditions and the study design includes replicates: several hives are observed under the same modality. Indexes are computed from at least a 10 x 10 section of capped brood cells for each hive and for several days during the brood development as a repeated measure.

In this case, a repeated measures ANOVA can be performed to compare results between negative reference and one or several test item modalities. The statistical design is a factor group (modality) and a factor time (repeated measures). Each hive is a basic unit. This statistical analysis permits one to assess factors as group factors but also interactions between factors which could be interesting for the experimenter to assess a slow rate in the brood development.

A second statistical approach is to perform the statistical analysis on the raw data of each cell. In every modality and every hive, each cell among the 100 selected cells is observed during the brood development. A quotation of the development status is assessed by the experimenter. Each cell is a basic unit. The statistical design is a factor group (modality), a factor time (repeated measures), and a factor hive. Multiple interactions between the factors can be computed and statistically assessed. This study design which includes each cell quotation in the statistics permits to increase the statistical power (statistical packages are available to perform this kind of analysis). ANOVA parametric or non-parametric without or with transformation on the data can be performed.