2.2. Strains

Various P. larvae strains can be obtained from bacterial culture collections. However, many of these collection agencies store exactly the same strains but with their own strain designations. This fact is relevant when comparing different P. larvae strains for research purposes, or when using strains as positive controls in diagnosis or species identification.  Table 2 shows many of the important strains that are available in these culture collections and shows some alternate designations. With regard to obtaining strains, it should be noted that certain countries have import/export and interstate transport shipping regulations regarding the movement and storage of this pathogen (http://www.biosafety.be/RA/Class/ListBact.html).

Table 2. Strains of Paenibacillus larvae.

Species

Old subsp. classification

Strain no.

Other designation

Source

Paenibacillus larvae

larvae

LMG 9820T

  ATCC 9545T

Foulbrood of honey bees

  DSM 7030T

 

  NRRL B-2605T

E C Holst #846

  LMG 15969T

 

Paenibacillus larvae

larvae

LMG 14425

  ATCC 25747

Ohio, USA, diseased honey bee larvae

Paenibacillus larvae

larvae

LMG 15969T

See LMG 9820T

See LMG 9820T

Paenibacillus larvae

larvae

LMG 16245

  NRRL B-3650

Diseased honey bee larvae

L Bailey, Rothamsted Expt. Station, Harpenden, UK. strain Australia (“Victoria”)

Paenibacillus larvae

larvae

LMG 18149

  Hornitzky 89/2302/4

Victoria, Australia, honey bee

Paenibacillus larvae

pulvifaciens

LMG 6911T

  ATCC 13537T

Dead larvae honey bee

  DSM 3615T

 

  IFO 15408T

 

  NCIMB 11201T

 

  NRRL B-3688T

 

  NRRL B-3685T

 

  NRRL B-3670T

 

  LMG 16248T

 

  LMG 15974T

 

Paenibacillus larvae

pulvifaciens

LMG 14427

  ATCC 25367

Unknown

Paenibacillus larvae

pulvifaciens

LMG 14428

  ATCC 25368

Unknown

Paenibacillus larvae

pulvifaciens

LMG 15974T

See LMG 6911T

See LMG 6911T

Paenibacillus larvae

pulvifaciens

LMG 16247

  NRRL B-3687

(1949), honey bee larvae, H Katznelson #754

Paenibacillus larvae

pulvifaciens

LMG 16248T

See LMG 6911T

See LMG 6911T

Paenibacillus larvae

pulvifaciens

LMG 16250

  NRRL B-14154

Unknown

Paenibacillus larvae

pulvifaciens

LMG 16251

  CCM 38

Unknown

  CCUG 7427

 

  NCFB 1121

 

  NRRL NRS-1283

Powdery scale, H Katznelson #113

Paenibacillus larvae

pulvifaciens

LMG 16252

 DSM 8443

Dead honey bee larvae

  NRRL NRS-1684