2.3. Sampling for AFB monitoring or diagnosis
Testing for the presence of P. larvae may be carried out for different reasons – for example either as part of national monitoring or prevention programmes, or as part of scientific research projects such as epidemiological studies. The proper collection procedure depends on whether the testing is to be carried out following the observation of suspected clinical signs of AFB, or the testing is part of general surveillance to identify a potential sub-clinical presence of P. larvae in colonies within a population. These considerations are addressed in the OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals vol.1 (OIE, 2008), and we advise readers to consult the AFB chapter in that resource.