Preparation of plates

  1. Dispense MYPGP-agar cooled below 50°C into sterile Petri dishes to give a level depth of 4 mm ± 0.5 mm (25 ml in 90 mm diameter Petri dish, 70 ml in 150 mm diameter Petri dish).
  2. Allow the agar to set before moving the plates.
  3. Prepared plates can be stored at 4-8°C in sealed plastic no more than 7 days before using.
  4. The surface of the agar should be dry before use.
    No drops of water should be visible on the surface of the agar when the plates are used. Plates must not be over-dried.