220.127.116.11. Sample collection and storage
- Grow at least ten independent P. larvae cultures for each condition to be analysed with qRT-PCR.
- Centrifuge cultures for 5 min at 8,000 x g and 4°C.
- Pour off the supernatant.
- Resuspend the bacterial pellet in RNAlater solution (Ambion).
- Incubate for approximately half an hour on ice.
- Divide the suspension in aliquots.
- Centrifuge aliquots for 2 min at 8,000 x g and 4°C.
- Store at -80°C.