5.1.1.1. Sample collection and storage

  1. Grow at least ten independent P. larvae cultures for each condition to be analysed with qRT-PCR.
  2. Centrifuge cultures for 5 min at 8,000 x g and 4°C.
  3. Pour off the supernatant.
  4. Resuspend the bacterial pellet in RNAlater solution (Ambion).
  5. Incubate for approximately half an hour on ice.
  6. Divide the suspension in aliquots.
  7. Centrifuge aliquots for 2 min at 8,000 x g and 4°C.
  8. Store at -80°C.