5.1.1.4. qRT-PCR reactions

For a single reaction, assemble the following components (total volume: 15 µl/reaction):

  • 7.5 µl 2x Platinum SYBR Green qPCR SuperMix-UDG (Invitrogen)
  • 0.03 µl of 100 µM forward and reverse primer each
  • µl cDNA template
  • µl distilled water