5.1.1.5. qRT-PCR program

Set program as follows:

  • 50°C, 2 min, 1 cycle
  •  95°C, 2 min, 1 cycle
  •  95°C, 20 sec, 40 cycles
  •  60°C, 40 sec, 40 cycles

         After PCR amplification, perform a melt curve analysis by measuring fluorescence after each temperature increase of 0.5°C for 5 sec over a range from 65°C to 95°C.