5.4.1.5. Clean up peptides

  1. From the final step in paragraph 5.4.1.4. ‘Label peptides with stable isotopes’, combine the two (for duplex labelling) or three (for triplex labelling) into a single tube.
  2. Repeat all steps from paragraph 5.4.1.3. ‘Clean up peptides’ above, eluting the peptides in step 5 into a 96-well microwell plate.