5.3. Anaerobic incubation

  1. Seal in an anaerobic jar as soon as possible with an anaerobic indicator (Oxoid BR055B) and Oxoid AnaeroGen anaerobic generator sachet (appropriate size for anaerobic jar volume. AnaeroGen AN025A for 2.5 l jar, AnaeroGen AN035A for 3.5 l jar).
  2. Incubate for about a week at 35°C.

The anaerobic indicator should go colourless from pink after a few hours incubation. If it does not, the jar has failed to achieve anaerobic conditions