7. Immunology-based methods

Various laboratory techniques based on the use of antibodies to visualize or distinguish between microorganisms exist. The key component in any of the vast array of methods used is the antibody. Polyclonal antibodies against M. plutonius can be prepared by injection of washed cultures of M. plutonius into rabbits either by intravenous injections (Bailey and Gibbs, 1962) or by a single intramuscular injection of 1ml M. plutonius suspension mixed with an equal volume of Freund´s incomplete adjuvant (OIE, 2008). Monoclonal antibodies can be prepared by injecting mice as described by Tomkies et al. (2009).