7.1.2. Sample processing

Individual or pooled samples of bees, larvae or pupae (sampled and stored at -20ºC) can be crushed in phosphate buffered saline, PBS, pH 7.4 (for the recipe, see Table 1 of the cell culture paper of the BEEBOOK  (Genersch et al., 2013)) , the homogenate centrifuged for 10 sec at 10,000 g and the supernatant stored at -20° C or used directly in an ELISA.