8.2.2. Larvae / pupae

Procedure:

  1. Place the larva / pupa in an Eppendorf tube.
  2. Add 0.5 ml grinding buffer (e.g. GITC).
  3. Ground with a micropestle.
  4. Centrifuge for 10 min at 7,500 g.
  5. Discard the supernatant.
  6. Resuspend the pellet in 180 µl enzymatic lysis buffer (DNeasy® Blood and Tissue kit, QIAGEN®).
  7. Use the Qiacube and the DNeasy® Blood and Tissue kit protocol for enzymatic lysis of Gram + bacteria for automated purification.
  8. Use the DNA templates directly in a PCR or store in –20 ºC until needed.