- Heat 5 ml of honey to 40 ºC.
- Mix thoroughly with an equal volume of PBS.
- Centrifuge at 27,000 g for 20 minutes.
- Discard the supernatant.
- Resuspend the pellet in the manufacturer`s
lysis buffer (DNeasy® Plant Mini Kit, QIAGEN®).
Follow the protocol for plant tissue (Mini Protocol).
- Use the DNA templates directly in a PCR or store in –20 ºC until needed.