8.2.3. Honey


  1. Heat 5 ml of honey to 40 ºC.
  2. Mix thoroughly with an equal volume of PBS.
  3. Centrifuge at 27,000 g for 20 minutes.
  4. Discard the supernatant.
  5. Resuspend the pellet in the manufacturer`s lysis buffer (DNeasy® Plant Mini Kit, QIAGEN®).
    Follow the protocol for plant tissue (Mini Protocol).
  6. Use the DNA templates directly in a PCR or store in –20 ºC until needed.