9.1.2. Total or microscopic count

A direct microscopic or total count is the enumeration of bacteria found within a demarcated region of a slide, a counting chamber. The slide is placed under a microscope, preferably with phase contrast. For counting bacteria, an oil immersion lens is usually required (1000 x magnification). For the procedure description refer to the section on hemocytometer counting in the miscellaneous methods paper of the BEEBOOK (Human et al., 2013).