Growth media

In culture, A. apis isolates can grow on many different media such as potato-dextrose agar (PDA), yeast-glucose-starch agar (YGPSA), and Sabouraud dextrose agar) (Bailey, 1981; Heath, 1982; Anderson and Gibson, 1998; Hornitzky, 2001), but they grow very well on media with high sugar content like MY-20 (Udagawa and Horie, 1974).


  1. Dissolve the following components in approximately 750 ml deionised water:
    • 4.0 g peptone
    • 3.0 g yeast extract
    • 200.0 g glucose
    • 20.0 g agar
  2. Adjust the final volume to 1000 ml.
  3. Autoclave at 115°C for 15 minutes.