1.4.3.2. Integral Rice Kernels (IRK) storage

  1. Autoclave (1 atmosphere and 120°C for 15 min) 25 g moistened IRK (2:1 wt/vol) in Petri dishes (9-cm diameter)
  2. Inoculate the IRK with 4-mm diameter plugs from the border of a growing agar colony
  3. Incubate at 30°C for 15 days
  4. Store the IRK at 19°C until use