1.4.5.2. GLEN medium

  1. Dissolve the following components in approximately 900 ml deionised water:
    • 4.000 g glucose-dextrose
    • 7.680 g natrium chloride
    • 6.500 g lactalbumin hydrolysate
    • 5.000 g yeast extract
    • 1.952 g MES-buffer (2-(N-morpholino)ethanesulfonic acid)
  2. Adjust the pH to 7.0 using 1 M NaOH
  3. Readjust the volume to 1000 ml
  4. Sterilize by autoclaving