188.8.131.52. Infection of colonies with spores in pollen
- Prepare a spore solution of 1.25 x 107 spores/ml in sterile distilled water. (See paragraph 1.4.3. and 1.4.4. Production and quality of inoculums)
- Add 100 ml spore solution to 150 g pollen
- Distribute the mixture on the top of the combs
An alternative method, if spore concentration is not very important, is to homogenize 15 black mummies and mix with 150 g pollen.