1.5.2.3. Infection of colonies with spores in pollen

  1.  Prepare a spore solution of 1.25 x 107 spores/ml in sterile distilled water.  (See paragraph 1.4.3. and 1.4.4. Production and quality of inoculums)
  2. Add 100 ml spore solution to 150 g pollen
  3.  Distribute the mixture on the top of the combs

An alternative method, if spore concentration is not very important, is to homogenize 15 black mummies and mix with 150 g pollen.