2.4.1. Isolation techniques

Aspergillus grows readily on many different standard media e.g. SDA, PDA, but Czapek-Dox medium, which contain sucrose as carbon source and nitrate as the nitrogen source should be very suitable. Czapek-Dox medium with addition of yeast extract (5.0 g/l) is recommended (Frisvad, pers. comm.). All these three media can be purchased premixed and easily prepared. E.g. to prepare 1l of SDA medium, suspend 65 g SDA (Merck) in 1l of demineralized water and autoclave 15 min at 121°C.