Fig. 7. Zone of inhibition assay of different compounds against Ascosphaera apis: A. MY-20 medium with spores spread on the entire surface and with a central hole for the test compound; B. The zone of inhibition was recorded daily by drawing on the Petri dish, a line over the border of the mycelium (each day a different colour) and the antifungal property was assessed as daily inhibition zone using an image analyser. Photos: J M Flores.