Giemsa staining

Giemsa’s stain stock solution is obtained from commercial sources. The prepared staining solution is prepared fresh by diluting the stock solution 1:9 using PBS buffer (pH 7.4).

  1. Apply smear onto microscope slide
  2. Allow to air dry
  3. Flood with 95 % methanol for 5 minutes, then pour off
  4. Flood with 10 % buffered Giemsa (pH7.4) for 10 minutes
  5. Wash carefully using tap water
  6. Dry using filter paper