220.127.116.11.1. Giemsa staining
Giemsa’s stain stock solution is obtained from commercial sources. The prepared staining solution is prepared fresh by diluting the stock solution 1:9 using PBS buffer (pH 7.4).
- Apply smear onto microscope slide
- Allow to air dry
- Flood with 95 % methanol for 5 minutes, then pour off
- Flood with 10 % buffered Giemsa (pH7.4) for 10 minutes
- Wash carefully using tap water
- Dry using filter paper