1. Centrifuge spore suspension at 5,000 G for five minutes to produce a pellet of spores. 
  2. Discard supernatant containing tissue debris that is lighter than spores,
  3. Resuspend the pelleted spores in distilled water by vortexing for five seconds

        Repeat the centrifugation 2-3 times to wash spores and to create a Nosema spore suspension with over 85 % purity level.