2.2.5.3. In vitro test for spore viability

In vitro germination of spores of both Nosema spp. can also be triggered by a procedure that mimics the natural conditions for the germination of environmental spores. Since the germination is the first step in the infection process, this test gives data on spore infectivity ability (Gisder et al. 2010b) but the in vitro test for spore viability has not yet been evaluated with the ultimate test - in vivo tests in live bees. See section 2.2.6. for details on in vitro rearing of Nosema.