Effects of different infection doses

It is necessary to test serial quantities of spores if the interest of the experiment is to investigate the effect of infection dose. In this case, for more precision, honey bees should always be individually fed the spore suspensions. The quantities of spores given to bees could increase by a factor of 10 (Forsgren and Fries, 2010; Malone et al., 2001), but other increments can also be used (Martin-Hernandez et al., 2011).