Fig. 7 A-C. Infection assay with Nosema spp. and primary pupal gut cells: A. infected primary ventricular cell; B. dissolving primary cell releasing new spores; C. in situ-hybridization (ISH) of infected ventricular cells, infected cells are stained blue. Specific hybridization was performed with 16S rRNA (SSU) probes coupled with digoxigenin and subsequent colour reaction to detect hybridized probes. Bars (A, B) represent 10 μm and bar in C represents 50 μm.