220.127.116.11. Individual rearing approach
- Fill standard 1.5 ml Eppendorf® reaction tubes 1/3 full with autoclaved pupation soil (see section 18.104.22.168.). Seal the tubes, place them in laboratory trays, and puncture the lids with a needle (3-4 small holes) to allow air circulation.
- Collect wandering larvae from the rearing program (see section 22.214.171.124.).
- Gently introduce an individual larva onto the soil of each tube using soft tweezers.
- Cover the larvae with another 1/3 full with autoclaved pupation soil (see section 126.96.36.199.).
- Seal the tubes.
-. Follow instructions for storage conditions of trays in incubators until adult emergence (see section 188.8.131.52.).
- Transfer adults, which have finished pupation (Fig. 3c), into new individual tubes.
- Provide with 1:1 honey water ad libitum
using a pipette.
The SHBs can be maintained for up to 8 weeks in darkness at RT.
If any other diet is used to rear or maintain SHB (e.g. to test reproductive success (Ellis et al., 2002b; Buchholz et al., 2008)), the food should be frozen first, especially if field collected (e.g. fruit) to kill any other insect eggs that may be present.