3.2.2.2. Identifying and marking brood cells in which SHB have oviposited

  1. Capped brood cells in which SHB have oviposited naturally or per the methods outlined in section 3.2.2.1. contain perforated cappings or side walls of the cell. The former is easy to note though the latter often requires a keen eye and the use of a flashlight.
  2. Mark capped brood cells exposed to SHB and containing perforated cappings and/or side walls using a transparent sheet of plastic (acetate).
  3. Cut the acetate to about the size of the face of the frame.
  4. Mark the sheet with a permanent marker in one of the corners of the frame to identify its correct placement on the comb in subsequent observations.
  5. Label the sheet according to frame and colony if several are used for replication.
  6. Once the acetate is stable on the frame, use a permanent marker to place a small dot on top over all brood cells containing perforated cappings and/or side walls.
  7. Once marked and labelled, remove the acetate sheet from the frame and store until needed.