3.3.1. Laboratory detection: microscopic detection of Acarapis woodi

The morphological technique most frequently used involves examining the prothoracic tracheae under a microscope.  Detection of low level infestation by A. woodi requires careful microscopic examination of tracheae.  When the infestation is heavy, the trachea will turn opaque and discoloured and mites can be noticed without the aid of a microscope. One method is to pull off the head and collar of a bee and examine the trachea (Sammataro, 2006; see video of bee dissection at: http://www.ars.usda.gov/pandp/docs.htm?docid=14370).