3.3.3. Sample preparation: morphological analysis and slide preparation
HBTM specimens should be preserved carefully for microscopic examination. Since, these mites are weakly sclerotized, no clearing agents are need. The mounting medium listed below is recommended for mounting specimens.
Mounting medium (Hoyer’s medium from Kranz and Walter, 2009)
50 ml of
30 g gum Arabic
200 g chloral hydrate
20 ml glycerol
Note: The ingredients should be mixed in the sequence listed above. Allow each solid ingredient to dissolve before adding the next one. The mixture needs to be warmed and stirred gently so the gum arabic can melt. Filter and store in airtight containers with rubber stoppers. Do not use a screw top container. Caution: chloral hydrate is a toxic chemical, a mutagen and a chromosome-damaging agent.