3.6.2.1.1. Mites to be used

What determines the end of the phoretic phase of mites is not known. Using phoretic mites for this assay is therefore not ideal since the randomly sampled mites might not be 'motivated' to enter cells and respond to the stimulus provided. It is therefore necessary to use mites which have recently expressed this behaviour and will repeat it again given the right circumstances. However, several experiments confirm that during the season most of the phoretic mites are willing to reproduce (e.g.  Rosenkranz and Bartalszky, 1996; Martin and Cook, 1996; Garrido and Rosenkranz, 2003; Frey et al., in preparation) and might also be used for these kinds of experiments (see section 3.1. ‘Collecting mites’ for a description of how to obtain them).