188.8.131.52.2.1. In the field
1. Treat brood cells in colonies with the stimulus under testing or treat mites.
2. Open cells a minimum of 70 h after mite introduction.
3. Investigate the presence of eggs in the cells.
Opening the cells later (e.g. between 7 and 10 days) will facilitate detection of eggs and offspring, but increase the chances of removal by hygienic workers.
Pros: less time consuming compared to laboratory bioassay (see section 184.108.40.206.2.2.). Many brood cells can be treated under natural conditions.
Cons: Stimuli cannot be tested independently from other factors (larvae, nurse bee activity).