220.127.116.11.2. Experimental setup
Donzé et al. (1998) used a modification of the bioassay described by Rickli et al. (1994) to study the chemicals inducing arrestment of the mite.
1. Use a glass plate cleaned with acetone and pentane.
2. Draw three concentric circles with 12 and 24 and 36 mm diameter on the underside of the glass (Fig. 9).
3. Apply the compound tested on top of the glass plate on the ring delimited by the 12 and 24 mm circles.
4. Place a mite in the centre of the circles (mites not reaching the treated area within 300 s are not considered).
5. Observe the mite’s walking activity.
6. Note the time spent in the treated area, this time is used as a measure of the arrestment activity of the stimulus under testing.
7. Stop the assay when the mite crosses the outer 36 mm
circle, or after 300 s.
Fig. 9. Test arena for arrestment bioassays.