10.2.7. Strand-specific real-time RT-qPCR

Finally, a very effective way to manage the consequences of illegitimate priming events during cDNA synthesis is to use real-time qPCR for strand-specific detection (Purcell et al., 2006; Gisder et al., 2009; Boncristiani et al., 2009; Plaskon et al., 2009; Lin et al., 2009; Zioni et al., 2011). This allows all the PCR products arising from rare cDNAs generated by false-, self- or mis-priming events to be excluded from the data on quantitative grounds.