10.3.2.2. Reverse transcription

1. Mix on ice:  
1.1.  10~500 ng         RNA,
1.2.  1 µl                   SALSA RT buffer,  
1.3.  0.5 µl       RT-primer mix,  
1.4. Sterile water to 4.5 µl total volume.  
2. Incubate 1 min at 80°C.
3. Incubate hhHeatjHggg5 min at 45°C.
4. Add 1.5 µl 20u/µL MuMLV Reverse Transcriptase.  
If necessary, dilute in 1:1 water: SALSA enzyme dilution buffer.  
5. Mix.
6. Incubate:
    6.1.  15 min at 37°C,
    6.2.  98°C for 2 min (reverse transcriptase inactivation),
    6.3. Cool to 25°C.