10.3.2.3. Hybridisation of MLPA half-probes

1. Add to the reverse transcription reaction and mix with care:
1.1.  1.5 µl     Probe-mix,  
1.2.  1.5 µl     MLPA buffer.  
2. Incubate:
  2.1.  1 min at 95°C,
  2.2. 16 h at 60°C in a PCR ThermoCycler.