10.3.2.5. PCR amplification of MLPA probes

1. Mix in new tubes:  
1.1.  10 µl               MLPA ligation reaction,
1.2.  4 µl                 SALSA PCR buffer,
1.3.  26 µl               sterile water.
2. While the tubes are in the thermal cycler at 60°C, add to each tube:  
2.1.  2 µl                 SALSA PCR primers,
2.2.  2 µl                 SALSA enzyme dilution buffer,  
2.3.  5.5 µl              sterile water,
2.4.  0.5 µl              SALSA polymerase.  
3. Incubate:
  3.1.  35 cycles [30 sec:95°C - 30 sec:60°C - 60 sec:72°C],
  3.2.  20 min:72°C.