5.6.2. Protocol

Here we describe a method for:

  • Producing full-length PCR products of positive-stranded, ssRNA honey bee viruses, with a T7 RNA polymerase promoter site incorporated into the forward primer sequence (sections 5.6.2.1. – 5.6.2.2.)
  • Cloning this product into a stable plasmid vector (section 5.6.2.3.)
  • Confirming the integrity and character of the full-length clones (section 5.6.2.4.)
  • Synthesizing full-length infectious RNA transcripts of the virus (sections 5.6.2.5. – 5.6.2.6.) 

 

5.6.2.6. Confirmation of Full-length Viral RNA