5.6.2. Protocol

Here we describe a method for:

  • Producing full-length PCR products of positive-stranded, ssRNA honey bee viruses, with a T7 RNA polymerase promoter site incorporated into the forward primer sequence (sections –
  • Cloning this product into a stable plasmid vector (section
  • Confirming the integrity and character of the full-length clones (section
  • Synthesizing full-length infectious RNA transcripts of the virus (sections – Confirmation of Full-length Viral RNA