Confirmation of Full-length Viral RNA

The full-length nature and activity of the transcripts is confirmed by in vitro translation experiments (Green and Sambrook, 2012), and ultimately by infection of pupae through injection with the synthesized RNA transcripts (see section 5.4). The infectivity of such transcripts is confirmed by comparing virus titres between transcript-inoculated pupae with control-inoculated pupae, and by sequencing the new virus infection (Benjedou et al., 2002).