9.3. RT-(q)PCR

The most common current methods for honey bee virus detection are based on Reverse Transcription Polymerase Chain reaction (RT-PCR), essentially the PCR amplification of cDNA. A detailed coverage of the principles and practices of PCR is found in Yuryev (2007) and the BEEBOOK paper on molecular methods (Evans et al., 2013). A reduced version, including those elements specifically relevant to virus detection, is presented here.