3.1. Natural rearing method

  1. Create a bee-free hive with frames of pulled, dark comb (dark comb is comb in which brood has been reared) containing honey and pollen.
  2. Introduce three, late instar larval wax moths per frame to ensure wax moth presence (Hood et al., 2004).
  3. The hive and combs should be covered and under some type of shelter to protect it from rain. Darkness, warmth, and lack of ventilation promote colonization.
  4. Unattended (bee-free) hives will be highly attractive to adult wax moths if they are present in the area (Hood et al., 2004)
  5. Provide additional used honeycomb containing honey and pollen as diet for rearing program as the food supply in the box is exhausted.
  6. Moth eggs, larvae, pupae and adults can be collected from the hive with an aspirator, forceps, or a small, soft paintbrush. The latter should be used for the immature wax moth stages since they can be damaged easily.