3.2. in vitro rearing of wax moths

Most in vitro lab rearing techniques follow a simple series of events:

  1. Place wax moth eggs on new diet.
  2. Allow resulting larvae to feed on diet.
  3. Harvest late instar larvae or pupa and place into a second container.
  4. Allow late instar larva to pupate or pupa to emerge as adults.
  5. Allow adults to mate and allow females to lay eggs.
  6. Place eggs on new diet.


Methods to accomplish these steps are described in subsequent sections.

3.2.8. Adult