3.2. in vitro rearing of wax moths
Most in vitro lab rearing techniques follow a simple series of events:
- Place wax moth eggs on new diet.
- Allow resulting larvae to feed on diet.
- Harvest late instar larvae or pupa and place into a second container.
- Allow late instar larva to pupate or pupa to emerge as adults.
- Allow adults to mate and allow females to lay eggs.
eggs on new diet.
Methods to accomplish these steps are described in subsequent sections.