If virgin females are needed, it is best to separate the females from males during the pupal stage as mating can occur shortly after adult emergence. The antennal and wing characteristics used to separate males and female adults (Table 1. and Section 2.4.) can also be seen in the pupal skins upon close examination. Smith (1965) provided two pupal characteristics which separate greater wax moth males and females:
- The mesowing demarcation has a notch in the apical margin of male pupal cases and is straight in female pupal cases.
- The sclerite of the 8th abdominal sternum is cloven in the female but not in the male pupal cases.