4.4. Quantification of wax moth populations in whole colonies

We could not find detailed instructions for quantifying the population of wax moths in living colonies. The default method would be to freeze the entire colony for at least one week, to ensure wax moth death, and then dissect the combs (section 4.3.) for careful inspection for and collection of the various wax moth life stages. This procedure likely could be modified by removing the bees from the colony (via shaking or brushing the combs) prior to freezing the combs. However, removing bees from the combs carries with it the added risk of shaking moth eggs, young larvae, or adults from the comb, thus making it impossible to quantify the moth populations accurately.