5.3. Incorporation of test compound into the wax moth artificial diet

  1. Prepare the treatment diet by adding the compound of choice to the wax moth artificial diet (Burges and Bailey, 1968; Eischen and Dietz, 1987). The diet should be prepared per Section 3.2.1. and the compound of interest added as experimental conditions necessitate.
  2. Obtain moth eggs are by creating an egg laying surface for mature females (per Section 3.2.5.) out of a piece of wax paper. The wax paper is folded back and forth, making tight folds (accordion style), and held closed on the end by a paper clip. The female moths will oviposit in the folds.
  3. Once eggs are laid, remove the folded wax paper.
  4. Tap the eggs into a vial with no food.
  5. As larvae hatch, move them carefully using a fine brush to a new vial provisioned with either treatment of control artificial diet.
  6. Monitor larvae can be for physiological change at set times throughout their development.

Considerations: First instar larvae are very small and quite active. It is important that lids to containers housing individual larvae and eggs be secured tightly to prevent escape. The egg container should be monitored regularly for newly emerged larvae. The first instar larvae will starve quickly without food, and larvae that emerge or die overnight should be removed from the container each morning.