5.7.3. Recovery of entomopathogenic nematodes from inoculated sand using greater wax moth larvae

Per (Fan and Hominick, 1991) and similar to the method outlined in 5.7.2., wax moth larvae can be used to recover entomopathogenic nematodes from inoculated sand.

  1. Prepare sand and plastic tubes according to the protocol outlined in 5.7.1.
  2. Inoculate the soil with any nematode species and/or any number of IJs of interest.
  3. Add single wax moth larvae (late instar larvae are 250-350 mg) to the soil.
  4. Keep the tubes at 20ºC.
  5. Replace the wax moth larva in the tube with a new individual weekly. This should be done until added larvae no longer die, indicating that no nematodes remain in the soil.
  6. Dissect all harvested larvae in saline or maintain on moistened filter paper at 20ºC for a period of time before use.