6.2.3. Removing haemocytes from haemolymph

Per Fröbius et al. (2001):

  1. Collect haemolymph from wax moth larvae per Section 6.2.1.
  2. Transfer the haemolymph to chilled tubes. The tubes should contain a few crystals of phenylthiourea to prevent melanization (Vilcinskas et al., 1997, Wedde, et al., 1998).
  3. Centrifuge the haemolymph twice at 100 g for 10 min to remove the haemocytes.
  4. Store the supernatants at -20°C until needed.