6.3. Eliciting immune responses in wax moth larvae

Per Wedde et al. (1998) and Fröbius et al. (2001):

  1. Suspend 20 mg zymosan A (Sigma) in 1 ml of sterile, physiological saline (172 mM KCl, 68 mM NaCl, 5 mM NaHCO3, pH 6.1, adjusted with HCl).
  2. Homogenize the mixture with a vortex.
  3. Centrifuge at 10,000 g for 5 min.
  4. Inject the supernatant and solubilized content at 10 µl supernatant/larva following Section 5.2.