6.4. Alternative method for eliciting immune responses in wax moth larvae

Per Schuhmann et al. (2003):

  1. Inject per Section 5.2. last instar larvae (250-350 mg) with 10 µl of bacterial lipopolysaccharide suspension (2 mg/ml in water; Sigma, Deisenhofen, Germany).
  2. Keep the larvae at 30ºC for desired amount of time.

Schuhmann et al. (2003) allowed them to sit for 4, 6, and 8 h – per desired experimental conditions.