6.5.1. Complete isolation of the larval cuticle

  1. Euthanize fully grown greater wax moth larvae with ether.
  2. Boil them in 5 % KOH for 3 h.
  3. Wash the larvae with water.
  4. Place overnight in 2 % pancreatin at pH 8.5 and 37ºC.
  5. Wash the remaining material with water.
  6. Remove any remaining tissues.
  7. Immerse the cuticle in boiling water for 20 min.
  8. Centrifuge to remove excess water.
  9. Dry the cuticle in a stream of hot air to constant weight