6.5.1. Complete isolation of the larval cuticle
- Euthanize fully grown greater wax moth larvae with ether.
- Boil them in 5 % KOH for 3 h.
- Wash the larvae with water.
- Place overnight in 2 % pancreatin at pH 8.5 and 37ºC.
- Wash the remaining material with water.
- Remove any remaining tissues.
- Immerse the cuticle in boiling water for 20 min.
- Centrifuge to remove excess water.
- Dry the cuticle in a stream of hot air to constant weight