6.7.1. Frame caging
Per Burgett and Tremblay (1979):
- Construct circular cages by replacing the metal sealing lid of 2 Mason® jar screw caps with 11.5 mesh/10 mm wire gauze and taping the two open sides together.
- Place contains diet medium (section 3.2.1.) in each cage.
- Place wax moths (life stage dependent on the project goals) in each cage.
- Secure up to nine cages, in rows of three, with large rubber bands in a standard “deep” Langstroth hive frame (480 × 29 × 230 mm; l × w × h) with no comb or foundation. Up to six cages, in rows of tree, can be secured to a standard “medium” Langstroth honey frame (480 × 29 × 160; l × w × h) with no comb or foundation.
- The wax moth frame may be inserted into a nucleus or
full sized brood box for trials.
Considerations: Frame caging is not ideal for bioassays involving eggs and early larvae. Dish containers (see Section 6.7.2.) are ideal for egg and early instar larval assays.