6.7.1. Frame caging

Per Burgett and Tremblay (1979):

  1. Construct circular cages by replacing the metal sealing lid of 2 Mason® jar screw caps with 11.5 mesh/10 mm wire gauze and taping the two open sides together.
  2. Place contains diet medium (section 3.2.1.) in each cage.
  3. Place wax moths (life stage dependent on the project goals) in each cage.
  4. Secure up to nine cages, in rows of three, with large rubber bands in a standard “deep” Langstroth hive frame (480 × 29 × 230 mm; l × w × h) with no comb or foundation. Up to six cages, in rows of tree, can be secured to a standard “medium” Langstroth honey frame (480 × 29 × 160; l × w × h) with no comb or foundation.
  5. The wax moth frame may be inserted into a nucleus or full sized brood box for trials.

Considerations: Frame caging is not ideal for bioassays involving eggs and early larvae. Dish containers (see Section 6.7.2.) are ideal for egg and early instar larval assays.